Judul : Bakteri Staphylococus sp.pada Hewan (Mikrobiologi)
link : Bakteri Staphylococus sp.pada Hewan (Mikrobiologi)
Bakteri Staphylococus sp.pada Hewan (Mikrobiologi)
Staphylococcus merupacan Gram-positive bacteria, 1 µm in diameter, Bergarombole seperti Ungab. The name derives from the Greek Bahasa "stafile" and "kokkos" untuk seikat ungrab dan berry.
About 30 species of staphylococci are found in the skin and mucous membranes, some of which cause pathogenic and purulent infections.
Most staphylococci are facultatively anaerobic bacteria and are catalase-positive, inactive, oxidase-negative, and Tidac mem-producing spores. Praying species include the subspecies S. aureus anaerobes and S. saccharolyticus are anaerobic and catalase negative.
S. aureus subspecies aureus (disebut juga S. aureus) and S. intermedius coagulase positive and S. hyicus coagulaseny varied and merupakan bacteri pathogen kekeh pada hewan home (1st table).
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| Gamber 1. The characteristic shape of staph is similar to staphylococcus |
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| Staphylococcus sp. (Image source: www.pinterest.com) |
Product coagulase berkorelasi dengan pathogenisitasnya. Although coagulase-negative staphylococci are usually low in virulence, some strains can cause disease in humans (Table 2).
Table 1. Positive Stahylococcus coagulase and Klinisnya advantages
(a) S. aureus can cause neonatal sepsis and lung infections in many species
(b) 20-50% of S. hyicus are coagulase positive
Table 2. Staphylococcus coagulase negative Yang Disolasi Dari Varabara Hewan
A) clinical and subclinical mastitis occasional dissection
residence
Staphylococcus sp. It deseruruh duna sebagai bacteria commensalis yang hidup di kulit hewan dan manusia. The bacteria are also found in the membranes that line the upper respiratory tract and lower part of the genitourinary tract.
Bacteria have a stable lignin state. Beberpas Staphylococcus menjukkan gekamanan Affinitas pada severa veena. Pemindahan de S. aureus between different species and between animals Dengan Manusia terbatas.
destination
- Coccus Gram positive, Yang bergerombol sukukta ungab
- Tumbuh Pada media tidak diperkaya (General)
- The size of the colony is medium, dengan warna putih or kuning.
- Colonization of S. areus and S. intermediaus leads to hemolysis
- Anaerobic in faculty, not mobile, but catalase positive
- Regular medical treatment, colic and colic
- Produksi koagulasenya bekorelasi dengan pathogenisitasnya
- Cenderung Stabil Dilingungan
- Menyebabkan pyogenic infection
Table 3. Gram-positive Pebedan bacteria coccus
walking: (a) Tests oxidase-fermentasi, O = oxidase, F = fermentativeDifferent types of staphylococci
Pada Sample Clinic, Staphylococcus sp. Micrococcus sp. Staphylococcus secara is overall catalase positive and Streptococcus catalase negative. Staphylococcus sp. Kidney colonies, qualitative hemolysis, profile biochemistry, RNA-ribosomal blocks of the Pol gene.
Some of the main Staphylococcus coagulase reactions are positive in Table 4. In some cases, especially in dogs and cats, it is important to know how to distinguish S. aureus from S. intermedius.
Figure 4 Differential Staphylococcus coagulase positive
go over
(A): 1% Maltose Pada Agar Ungu
(B): Prevention of intentional poisoning
(c): anaerobic
Later: Not available
+: up to 90% positive species
- Up to 90% negative stress
+/-: pemanfaatan kurang
Q: Answers vary
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| Account Colon Yang Tumhub Pada Media Sheep Blood Agar (Dark Gambar: www.microregistrar.com) |
There is no diagnostic drug in the laboratory, the label is different, staph coagulase negative is usually based on an almost pure culture that is isolated, or on a body in a normal place such as blood or cerebrospinal fluid.
1 character colony
Colony Staphylococcus usually Bewarna Putih, Buram, Diameternya Hingga 4 mm. During the colonization of Staphylococcus manusia and Sapi adalah Kuning Kultman. Based on some staphylococcal coagulase negative Juga berpigmentasi (Bewarna) species.
2. Hemolysis pada domba or juicy bloody agar
Diketahui Ada Impat Hemolysin Dari Staff, Yaitu Alpha, Beta, Gamma and Delta. Each type of hemolysin differs in several ways, such as antigens, biochemistry, and effects.
The ability to produce hemolysin varies between different strains, and strains of S. aureus and S. intermedius usually produce alpha and beta hemolysins.
Pada agar darah ruminansia, alpha hemolysin zone, tipis yang nigimakan zone hemolysis causes complete decimate colony, and beta hemolysis zone is called partial yang-lebih-luas or incomplete hemolysis. This condition is associated with hemolysis in the blood (Section 2).
Hemolysin Hemolysin Terseb Bertidak Sebagai Toxin Pada Test In Vivo. Staphylococcus coagulase negative variants have the ability to produce hemolysis, which is usually slow. Isolas Dari S. Haikus tidak bitang hemolcci is not hemolytic.
3. Coagulase Pad Kaca Preparation and Tube.
Pada test Tersebut, Sebuah Suspensi Staphylococcus di Campur Dengan Plazma Kelinci Baik Pada Kaca Dissection microscope or small tube. Fibrinogen is then converted to fibrin by coagulase:
- Pada kaca preparation didetksi andanya forced coagulation or atau cause Penggumpal Pada surface bacteria. If the reaction is positive, the bacteria will be collected in 1-2 minutes.
- Mendetexi fights plasma bacteria with coagulase or staphylocoagulase. Ini merupakan test to determine tes coagulase. If the reaction of the product shows a positive reaction, the mold is placed in a tube at 370 cm for 24 hours.
4. Biochemical tests for mensekkan S, Aureus and S. intermedius
- Uji Sepat (Fasting) Dectari Anandaya Pembucan Acetoin
- Agar powder (purple agar), pH and 1% maltose indicator, S. aureus dari S. intermedius. Staphylococcus aureus uses maltose and Assam productije perawakan pada medium and colony ungu menjadi kuning. Although Staphylococcus intermedius is not good at fermenting maltose, it does not change the color of the medium.
- Biochemistry can be intoxicated, which does not confirm the difference from staphylococci.
5. Molecular processing
Polymerase Chain Reaction (PCR) Research Laboratory.
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Gambar 2. Characteristic hemolysis Ganda S. aureus from S. Intermedius Pada lamb/beef blood white. |
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| Comparative hemolytic background. 1 (accession number: delrio.dcccd.edu) |
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| Comparison hemolisa darah 2. (Summer Gamber : pemburumikroba.blogspot.com) |
pathogens and pathogens
Since staphylococci are pyogenic bacteria, the bacteria that cause the disease are pure. Simple injuries or infections that cause immune problems. The virulence and pathogenicity of S. aureus are presented in Table 5.
The relationship between pathogens and various factors is still unknown. But some virulence agents such as plasmid or phage termidia, Diduga Menjadi Penetu virulence and Penyandi genes Staphylococcus.
Structural bacterial capsular polysaccharides, teichoic acid and protein A interfere with or inhibit oposcinization and phagocytosis.
Protein salt is staphylococci, fibronectin and fibrinogen, microorganisms and bacteria.
Coagulase-producing Staphylococcus is an indicator of pathogenic bacteria. Dynase and protein A production are activated.
investigation process
- Epidermatitis Exudative Pada Baba Muda and Tick Pyamia Pada Domba Muda Adalah Hanya Some Condition Clinics Pada Hewan Domestica, Khusini Diakibatkan Oleh Staphylococcus Pathogen. Purulent pada disease, sepertic staph infection and menimbil sample, special sepertic exudate and milk mastitis yang diliman untuk process laboratory is considered.
- Penampakan koloran gram pada nanah ataw pattern line yang bhasakan dapat nimuhakan type step.
- Try Yang Diculture Pada-Agar Darah, Agar Darah Selector, then Maconkey Agar and incubation in aerobic Pada Suhu 37 C Dalam 24 Hingga 48 Jam. If you can choose Yang menending asam nalidiksik and colistin, bacteria Proteus sp. Pollutant gram-negative Lanya.
- bacterial isolation criteria;
- behavioral colonization
- Ada tidaknya hemolysis
- The product is catalase
- coagulase production
- Profile of Biochemistry
- Phage-type bacteria fight staphylococcal infections during epidemiological studies.
Table 5. Virulence factor including Staphylococcus aureus toxin and pathogenicity.
At the fax clinic
Since staph, as commensal bacteria, act on everything from coatings to banana straws and skin as environmental contaminants, infection can generally be internal or external.
Traumatic, immunological, allergic and hormonal metadata or infestations. Staphylococcal coagulase has a good effect. (See Table 1)
Some species Mameliki virulencei Renda, Dengan coagulase negative Juga Mapu Melamada Penyakat Pada Free Hewan (Table 2).
The vaccine is currently available. Uji's sensitivity to antibiotics should be done first before treatment.
Penyakit Staphylococcus yang pentang pada hewan domestick yaitu Mastitis, tick-borne pyaemia, exudative epidermatitis, botryomycosis and pyoderma.
Pada sapi staph mastitis.
Staphylococcal mastitis, often caused by S. aureus, or other common causes of mastitis. Penyakit ini dapat besifat subclinical, acute atau, chronic, namun Most infections are subclinical.
Bentuk perakut dab gangrene systemic reaction yang parah va dapat menagat niya hewan swallow. Pada mastitis gangrin ada impat hal yang terka, yatu tubuh menjadi dingin dan be warna biru khitamaan, pada asirnya terkelupas.
The tissue undergoes necrosis caused by alpha toxicity, which causes contraction that causes necrosis of the smooth walls of blood vessels, which disrupts blood flow.
In addition, lysosomal leukocytes are an enzyme for releasing toxins. Mastitis akan discusikan lebih langjut deline waktu.
Tick-borne pyaemia
Tick-borne pymia, Adalah infecti yang Berudana s. Aures pada domba muda, yang besparu pada willaha gembala perbukitan d'engris dan irlandia, yang merupakan yang kok untuk kaplak kaplak kaplak Ixodes ricinus.
Domba muda can carry S. aureus from the skin to the nasal mucosa, where infection occurs through minor skin lesions and itching. Ixodes ricinus merupakan ventor untuk penyakt ricketsia dari tick fever, Ehrlichia fagositopila, yang dapat melamendan immunosupresssi pada domba muda and melamendan predisosisi pada infection staphylococci.
Tick lice Pyemia karakterisasi baik oleh septicemia and death cepat en berasasan abscess yang terlokalisasi pada banyak organ.
Clinical manifestations include arthritis, paresis of the back. On some farms, where 30% of lambs are 2 to 10 minutes old, the disease is having a significant economic impact.
investigation
- Pada domba muda, yang berkliaran di padang gembala yang rimbaun d ingris atau irelandia, segman klinis dapat dikurigai terka penyak ini.
- Bacterium Pada nana from Gambaran microcopies based on isolation and identification of S. aureus lesions.
treatment and administration
Therapy terbatas nilaniya pada matter domba yang sangat banyak terka penyakat. So the handling should be direct, then mamasukan merca ke dalam kandang, dan mengobati merca di dalam kdan pula.
- Therapy Prophylaxis Pada Domba Muda Dengan Antibiotics Seperti Tetracycline long-acting, Dapat Mengoinicia Dalam Satu Mangu. Tetracycline Juga Dapat Kututya Domba Muda Dari E. Phagocytophila
- Pengendalian kaplak seperti pemandian dalam kolam yang sadah is disinfected (immersion 0 should be done)
Exudative epidermatitis
Penyakit ini, due to S. haikus, yang tadida diseruruh dunya pada babi muda hinga bemur 3 bulan. Penyakit ini sangat menu and sebum secretion berlebih diseruruh tubuh, exfoliasi, then exudasi pada sudusat kulit characteristic.
Affected children have anorexia, depression, dental fibrillation, widespread swelling, nonpruritic dermatitis with exudative exudate. Babi mda dibawah 3 mingu dapat mati dalam 24-48 jam.
Morbidity ranges from 20-100% and mortality can reach 90% in affected litters. Staphylococcus Hycus Dapat Disolasi Dari Mucosa Genitalia Dari Babi Bettina Dowasa Yang Sehat. The body is believed to be Masuk of Kulit Babi Muda Malaui Abrasi of Kulu Seperti Luca Gigitan.
Causal predisposition sepert stress, diakibatkan karena induk tidak dapat prodiyiji susu lagi, dan mempararah teradainan infection. Research Studies, Toxin S. hyicus dapat manakibatkan ekfoliasi.
investigation
- Tinginya Mortality Pada Babi Muda Dengan Expository, Lacy Pada Kulit Non-Prutitic (tdak gattal) adalah siri kas penyakat in
- Isolation and identification of S. hyicus dari lesi kulit for confirmation.
treatment and administration
- If possible, antiseptic and antiseptic antiseptics and antiseptic systems antibiotics.
- Isolation of infant patients
- Bersihkan and insecticide Bungan Young Reontaminasi
- Babi Bettina Dowasa Berehng in the background dengan sabut antiseptic
- Utamakan colonisaci pada kulit dungan stame avirulen s Hycus untuk jaw infection s Hycus young virulent secara experimental allele.
botryomycosis
Chronic disease botryomycosis, purulent granulomatous disease proving Disebakan oleh S. Aureus.
Penyakit ini dapat tadirikan dalam niguri negugu seketele castrasi pada kuda yang yang oleh infection pada korfa spermatica.
Botryomycosis Juga Dapat Tadari Pada Tiskaran Mamaye Dari Babi Bettina Dowasa. Lesi Derupa Fibrous Mass and Jarringan Mend Nanah.
Staphylococcus infection in cats and dogs
Staphylococcus intermedius General Diseolazi Pyoderma, otitis externa, Nies and Lanya diseases, acute mastitis, endometritis, cystitis, osteomyelitis and other infections. Occasionally, purulent conditions can also occur with S. aureus.
referee
Quinn PJ, Markey BK, Carter ME, Donnelly WJ, Leonard FC 2003. Animal Microbiology and Microbiological Diseases. Blackwell Science Editor.
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