Judul : Media Pertumbuhan (Buatan) Mikrobiologi
link : Media Pertumbuhan (Buatan) Mikrobiologi
Media Pertumbuhan (Buatan) Mikrobiologi
A. Pengertic Media
Interpretations of Pengertian Dan in Pertumbuhan media
Growth medium for microorganisms
Microorganisms use nutrient pada media molecule purapa molecule kechil yang dirakite untuk meninu compeon sel-nya. By the way, according to media reports, the microorganism becomes Mengelsolovsky, but the cultures are the same.
The composite medium is formed by various types of isolation and separates the microorganisms that make up the mass.
Media adalah suatu bahan yang tadashi dari missik zat-zat hara (food) yang medida untuk membyakan microbe. Dengan mempergunakan kungang-makam media dapat dokanan isoliza, perbanyakan, tesing sifat-sifat physiologis dan exanakan sumum microba (Sutejo, 1996).
B. Manfat dan funsi media
The medium acts as a source of food, nutrients, and nutrients for the microorganisms growing in the medium, as well as the Membiakan, Mengasingkan, Penyakan, and Meimpan microorganisms.
Media juga dapat dibukan untuk sifat-sifat colony/pertumbuhan microorganisms, serta sifat-sifat biochemical.
In the laboratory, microbiological drug media can be used to carry antigens, toxins, and microbes for virulence and similar transformations.
GRAM. Persian media
To replace favorable environmental conditions with natural conditions, certain conditions are necessary for the normal growth and development of bacteria in the environment. See Persian thersbut.
- The medium may contain the growth and development of bacteria.
- The medium must have an osmotic pressure, surface tension and pH in accordance with the needs of the bacteria.
- The medium must be sterile, that is, the bacteria in question do not come into contact with other microbes.
e. Build Pertumbuhan
1. Tingkat Kesaman (pH)
Most of them have a neutral pH, and a low pH of 4.6 - 7.0 is suitable for the release of bacteria, and it is higher than the pH of the chamber.
2. Dry
Suhu Merupakan is one of the environmental factors affecting the growth of microbes. Each microbe has a small size and a suitable temperature for its growth.
Microbes are found in small groups due to growth at low temperatures.
- Psychrophiles, that is, microbes that grow at a temperature of 0-20 degrees Celsius.
- Mesophile, a microbe that grows at a temperature of 20-45 degrees Celsius.
- Thermophilic, temperature 45 degrees Celsius.
Most microbes in mesophyll microbes perusak pangan merupakan, yaitu tumbuh baik pada suhu borung atau suhu kamari. Pathogens usually have a good growth temperature of 37 degrees Celsius , which is the temperature of the human body.
Oleh karena itu suhu tubuh manusia merupakan suhu yang baik untuk growth of some pathogens. Microbes and pathogens grow at low temperatures of 4-66 degrees Celsius .
3. Nutritious food
Microb sama dengan qulukuk hidup lainnya, nekukan supply nutrisi sebagai sumber energi dan fugasu selnya. Unsur-unsur dasar tersebut adalah: Carbon, Nitrogen, Hydrogen, Oxygen, Sulfur, Phosphorus, Zat Besi Dan Janga Kesil Logam Linea.
Ketidaan atau lack of nutrient source in ini dapat affects the growth of microbes, which eventually leads to death. The condition in the environment is unhygienic and unhygienic, which is a condition that gives microbes until finally leads to death.
However, the principle of keeping the environment clean and hygienic is maintenance and the minimum amount of nutrisi bagi microba agar fugatsunya terkendali.
4. Oxygen
Microbes have different oxygen requirements for growth. According to the need for oxygen, microbes are divided into 4 groups.
- In football, these will be aerobic, micro and oxygen.
- Anaerobic microbe yaitu yan tukhum tanpa mekbu oxygen.
- Anaerobic facultative, microbial values and oxygen should be given.
- Microaerophiles are microbes that require less oxygen than the normal amount of oxygen in air. Microbe perusak pangan sebagai besar classia aerobic, yaitu secrembe oxygen untuk futsangnya, gepah bacteria yang dapat tumbuh pada kalsana salidini manusia yang classia anaerobic facultative.
5. Pressure osmosis
Suatu pentagonal osmosis, akan sangat in Luzhany bacteria, pentagonal osmosis, leiniger lebig besar (hypertonus), cellular akan temenin plasmolysis.
On the other hand, osmosis pressure lingeringan yang hypotonis akan membengkak sel membengkak dan juga dapat membangan rusaknya sel.
Oleh karena itu dalam mendasana languya, cell bacteria harus berada pada tingga yang convincing osmosis pressure, cell bacteria memeliki diurnal adaptation, perdamanan osmotic pressure dengan lingkugannya tidak boleh tereai besar.
6. Infertility
The medium must be sterile, that is, the bacteria in question do not come into contact with other microbes.
E Bahan Pembuatan Media
1. Bahan Dasar
- Air (H2O) as:
- Agar (dari high romput) yang funjaksi untuk pemadat media. Agar Sulit Degradasi Oleg Microorganisms Pada Jimune and Menker Pada Suhu 45o C.
- Gelatin mushrooms and agar. Gelatin is a polymer made from collagen. His disadvantage is more than a partner.
- Silica gel, sodium silicate. Fungsinya juga sebagai pemadat media. Microorganisms are obligate autotrophs in silica gel protected media.
2. Nutrici or Zat Makana:
Media Harus mengagung unsur-unsur yang languar untuk metabolism sel yaitu perupa unsur macro seperti C, H, O, N, P; Our micronutrients include Fe, Mg and our Pelican/Trace nutrients.
1) Carbon saber
Organic molecular carbon, carbon, carbohydrates, carbohydrates, proteins, glucose, DL and cells. These are organic carbon-forming organisms, as well as heterotrophic and microbial organisms.
2) Total nitrogen
Sumer Nitrogen Menkakup Asam Amino, Protein Atau Senyawa Nitrogen Line Young Tercanden Pada Peptone, Meat Extract, Atau Tryptose. Sejumla microba juga dapat menggunakan sumber N anorganik seperti urea.
3) Summer oxygen
Diculture of heterotrophic microorganisms pada kawan, sebagian besar getapatkan langsung dari blow microorganism diculture pada kare samber oxygen belamandari oxygen yang terlarut air.
Oleh karena itu aerasi pada culture cair dapat microorganisms increase oxygen supply.
4) permanent phosphate
Sumber is an organic phosphate stabilized protein, cofactor or ATP or yeast extract cofactor or peptone. Namun hampi semua microorganisms dapat
Use of inorganic phosphates added to septrialium phosphate, sodium phosphate, etc. (Prescott and Harley, 2002:98).
5. Sumber unsur sekelumit (microscope/microelement).
In the environment of the medium in a petri dish, a trace element element (Zn, instrument glass), the function of a trace element is generally to be part of an enzyme or cofactor to stimulate a reaction or maintain the structure of a protein.
Oleg kerena itu pembuktian kehogan usur microeutrien sangat promises kikanda dalam scala laboratory kerena yap genus sekebinia microorganism dalam janyan sangat sedik (Prescott and Harley, 2002:96).
3. Bahan Temba
- For certain purposes, additional substances are added to the medium, for example, phenol red (assam base indicator) is added to the pH change indicator.
- Antibiotics increase non-target/contaminant microbes.
4. Bahan Yang Sering used cotton fabric.
- Agar can be found in the form of sticks, granules, or powder, and is made from a variety of plant species. Kegunaanya adalah sebagai pemadat (gel) yang pertama kali diwana oleh Fraw and Walter Hesse for the media. If it is mixed with cold air, it will not dissolve. Untuk melarutkanya harus diasuk dan dipanasi, penkairan dan pemadatan berkali-kali atau sterilesasi yang teragi lam dapat menkankan ekshatan agar, especially pada yang ph yang.
- Peptone Peptone produces hydrolyzed proteins including liver, liver, whey, casein, lactalbumin, gelatin and algae. Composinia takang pada bahan cough and bagimagan kara perternya
- Meat extraction Choice of organic meat, spleen, placenta and meat.
- Yeast extract Yeast extract Terbuat Dari Ragi Pengembang Roti Atau Pembuat Alcohol. Yeast extract Menganden Asam Amino Yang Lengkap and vitamin (B complex).
- Hydrocarbon amino and hydrocarbon gases are attached to carbohydrates. GENESIS CARBOHYDRATES AND GYMUNCAN DIGUNCAN DALAM AMYLUME, glucose, fructose, galactose, sucrose, mannitol, DL. Concentration 0.5-1%.
MAKAM MAKAM Media:
Media untuk bacterial culture dalam microbiology ada banyak jenisnya dan dapat menjadi tiga kelopus besar basan sukuk, compposizi/susunnaya.
A. Bentucnia based
Bentuk media ada tiga makam yang dapatdiverdakan dari ada atau tidaknya bahan exaban purapa bahan pemadat seperti agar-agar or gelatin. Bentouk Media, Thursbut, Yaitu.
1. Media panel:
Media padat merupakan media yang mengagung banyak agar atau zat pemadat kurang lebih 15% agar sehaga media menjadi padat.
This medium can be divided into three types according to its shape and container: solid medium, meringue medium and flat medium.
media tega mengunan tabung reaksi yang ditegakkan sebagai vadahngna, media miring mengunan tabung rexi yang dimeringkan, media limpg mengunan petridik (tri) sebagai vadahngna.
Medium ini giummenti dinukum untuk futsang bacterial colony atau kapang kalau ke dalam media addidan antara 10-15 g Tepung agar 1000 ml medium in. Jumlah tepung agar-agar yang addidan kepakan kepaka jenis atau kelomp microbe yang nidara.
Kalau ke dalam media tidak addindum zat pemadat, Jimunen sikken untuk pembiakkan microalgae, as well as other microbes, especially bacteria and yeasts. Ada yang nekukan kadar air tinggi seguh jamma tepung agar-agar renha.
But there are pools that need kangungan air rendah sehatan tepung tepung agar haru sedi. Medium for bacteria, microalgae Ragi, Jamur and Kadang-Kadang Juga
2. Half media
Media Medium regime or middle class media Media 0.3%-0.4% 0.3%-0.4% Thus, the media will be Kenyan.
It is used to grow many microbes that normally require air and anaerobic life to see microbial activity.
Kalau zat pemadat hanya 50% in your kurang dari yan suridu. Ini gyumteni kalkuri untuk futsang microb yang banyak sikkem tepadan air and hidup anaerobic and selective.
3. Media
Media kair merupakan media yang tidak kopasati bahan pemadat, gyumtuna dibukan untuk futsang microalgae. Kalau ke dalam media tidak zat pemadat is added, usually microalgae is used in pembiac, but other microbes, especially bacteria and yeast.
b. Based on composition / Susunanya
Based on media composition by Bagi Atas.
- Natural/non-synthetic carriers are carriers composed of naturally occurring materials whose composition cannot be determined with certainty and are usually derived directly from the base material. kentang, tepung, daging, telor, ikan sayur, tomato juice agar.
- Semi-synthetic carriers merupakan media yang severagan give natural materials and synthetic materials. Contains the following: Kaldu nutrisi saravanje dari: Peptone 10.0 g, Daging Extract 10.0 g, NaCl 5.0 g and Aquadest 1000 ml.
- Media Synthesis, Yaitu Media Jan Semparia Dari Segnava Chemistry Yandin Dan Skanya Diketahu Sekara Pasti. For example: MacConkey agar.
depending on the form
- Media Alami, Yaitu Media Jan Sempari Oleg Bahan-Bahan Alami Seperti Kentang, Tepung, Dagung, Tulur, Ikan, Umbi-Umbian Lainya and Sebgaina. Pada saat sekaran
- Media Alamy Jan Banyak Dicolor Adalah Dalam Culture Yaringan Thanaman Kumanam Havan. Microorganisms in the environment contribute carbon to the biomass. Kalau ke dalam media tidak zat pemadat is added, usually microalgae is used in pembiac, but other microbes, especially bacteria and yeast. According to media reports, canine and viral immunity in football.
- Media Blend Media can be a blend between media and others.
- K2HPO4: 0.5 g
- KH2PO4: 0.5 g
- MgSO4, 7H2O: 0.1 g
- NaCl: 0.1 g
- FeSO4, 7H2O: 0.01 g
- MnSO4, 7H2O: 0.01 g
- CaCO3-Seangin
- Semi-synthetic media yaitu media yan tersun oleh kanpuran bahan-bahan alami dan bahan-bahan synthetics.
Here are some common environments in microbiology.
A. Lactose soup
Lactose broth sebagay media untuk dextari seheda E. coli dalam air, makanan, dan product susu, sebaga kaldu pemerkaya (pre-grated broth) untuk is used.
Salmonella and Dalam Delmenada Fermentasi Lactose Ole Bacterium Pada Jimuni. Peptone and meat extract contain important nutrients for bacterial metabolism. Lactose is another carbohydrate source for coliform organisms.
b. EMBA (eosin methylene blue agar)
Eosin-methylene blue medium contains lactose and agents, microbes, and lactose-producing pathogens S. aureus, P. aerugenosa, and Salmonella.
Microbes called memfermentase lactase produce black color with chelated iron. In the Yang Dapat Tumbukh Tidak Berwana colony, where the microbe sleeps.
in. nutrient agar
Nutrien agar adalah creates an environment with air and milk. He simply chooses microorganisms, and these are heterotrophic microorganisms.
This medium is a simple medium prepared from serum, peptone and agar. The media distinguish bacterial processes used in media, air, wastewater, producers, crop cultures, other bacteria, and other cultures of organisms.
It. Balanced Soup
The microorganisms are returned to nutrient broth media. Intinya Sama is informative.
e. MRSA (de Manna Rogosa-Sharpa agar)
According to the media reports on MRSA presented by de Man, Rogosa and Shapp (1960), lactobacilli are not beneficial.
MRS Agar is a mixture of polysorbate, acetate, magnesium and manganese of lactobacilli, nutrient dipercyte.
f. Trypticase Soy Sauce (TSB)
TSB adalah media salda dipercaya tujuan umu, isoliza and penumbuhan for various microorganisms.
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